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زیست شناسی::
پروموتر پلیهدرین
In these cells, the polyhedrin promoter is exceptionally active, and during infections with wildtype baculovirus, high levels of polyhedrin are synthesized.
The AcMNPV DNA upstream from the polyhedrin promoter (5?
The transfer vector is an E. colibased plasmid that carries a segment of DNA from AcMNPV (Fig. 3.35A) consisting of the polyhedrin promoter region with flanking upstream AcMNPV DNA, a multiple cloning site, the polyhedrin transcription termination and polyadenylation signal regions with flanking downstream AcMNPV DNA.
A gene of interest is inserted into the multiple cloning site between the polyhedrin promoter and termination sequences, and the transfer vector is propagated in E. coli.
Within some of the doubly transfected cells, a doublecrossover recombination event occurs at homo logous sequences on the transfer vector and in the AcMNPV genome, and the cloned gene with polyhedrin promoter and termination regions becomes integrated into the AcMNPV DNA (Fig. 3.35B) with the concomitant loss of the polyhedrin gene.
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